Multiplex PCR: Optimization and Application in Diagnostic Virology
نویسندگان
چکیده
منابع مشابه
Multiplex PCR: optimization and application in diagnostic virology.
PCR has revolutionized the field of infectious disease diagnosis. To overcome the inherent disadvantage of cost and to improve the diagnostic capacity of the test, multiplex PCR, a variant of the test in which more than one target sequence is amplified using more than one pair of primers, has been developed. Multiplex PCRs to detect viral, bacterial, and/or other infectious agents in one reacti...
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The use of the polymerase chain reaction (PCR) in molecular diagnostics has increased to the point where it is now accepted as the gold standard for detecting nucleic acids from a number of origins and it has become an essential tool in the research laboratory. Real-time PCR has engendered wider acceptance of the PCR due to its improved rapidity, sensitivity, reproducibility and the reduced ris...
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ژورنال
عنوان ژورنال: Clinical Microbiology Reviews
سال: 2000
ISSN: 0893-8512,1098-6618
DOI: 10.1128/cmr.13.4.559